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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is validating a new automated blood culture system. To assess the system's ability to detect true positive results, they should:

A) Evaluate the system's performance with samples from patients with known bloodstream infections.
B) Spike known concentrations of various microorganisms into blood culture bottles.
C) Compare the time to detection with the current manual blood culture method.
D) Process a large number of sterile blood samples.

2. A microbiology laboratory is asked to identify the causative agent of a fungal keratitis. Microscopic examination of corneal scrapings reveals broad, aseptate hyphae with right-angle branching. The MOST likely genus is:

A) Aspergillus
B) Fusarium
C) Candida
D) Mucor or Rhizopus

3. A patient develops a febrile illness with jaundice and dark urine after receiving a blood transfusion.
Laboratory testing reveals an elevated level of unconjugated bilirubin and a positive direct Coombs test.
Serological testing is positive for antibodies against a protozoan parasite that infects erythrocytes. The MOST likely causative agent is:

A) Babesia microti
B) Trypanosoma cruzi
C) Plasmodium falciparum
D) Toxoplasma gondii

4. A clinical microbiology laboratory is implementing whole-genome sequencing (WGS) for enhanced surveillance of Listeria monocytogenes isolates associated with foodborne outbreaks. WGS provides several advantages over traditional subtyping methods like pulsed-field gel electrophoresis (PFGE). Which of the following is the MOST significant advantage of WGS in this context?

A) Faster turnaround time for obtaining results.
B) Easier standardization and inter-laboratory comparison of data.
C) Higher discriminatory power for resolving phylogenetic relationships and identifying outbreakclusters.
D) Lower cost per isolate compared to PFGE.

5. A clinical microbiology laboratory isolates a Gram-negative rod from a wound infection following a dog bite.
The organism grows on MacConkey agar, is oxidase-positive, and produces a characteristic bleach-like odor.
The MOST likely identification is:

A) Pasteurella multocida
B) Capnocytophaga canimorsus
C) Bordetella bronchiseptica
D) Pseudomonas aeruginosa

Solutions: